Production of alpha amylases by aspergillus niger using cheaper substrates employing solid state fermentation jahir alam khan1 and sachin kumar yadav2september 2010. The 2 agroindustrial byproducts maltose and saccharose, and also lactose and starch were individually evaluated for. Amylase production by aspergillus niger under solid state fermentation using agroindustrial wastes. Production of fungal amylases using cheap, readily. The optimum ph, temperature and incubation period for enzyme production were 5. Wheat bran is proved to be a good substrate for the alpha amylase production by a.
Characterization of extracellular amylase enzyme produced by aspergillus flavus. Amylase production by aspergillus niger through submerged fermentation using starchy food byproducts as substrate jiby john mathew, prem jose vazhacharickal, sajeshkumar nk and anjaly ashokan abstract submerged fermentation holds tremendous fungal. Pdf amylase production by aspergillus niger under solid state. It is also used as an additive in soaps, detergents and animal feeds, and in the treatment of paper, textiles, etc. Aspergillus niger is cultured for the industrial production of many substances. Go, production of amylase by aspergillus niger in a cheap. The enzyme comprised of a typical secondary structure containing. Production of amylase from fruit peel using aspergillus. Starch depolymerisation by amylases is the basis for several processes such as glucose syrups production, bread making and brewing. Pdf amylase production by aspergillus niger through submerged. Production and optimization of amylases using aspergillus. Production of raw starch degrading amylase by a mixed culture of aspergillus niger and saccharomyces cerevisae grown on sorghum pomace as nutrient source was investigated.
Mjsu1101 later identified as aspergillus niger was. Production and optimization of amylases using aspergillus niger. Nirmala department of biotechnology sathyabama university chennai 600119 india skrn. Data pdf available may 20 with 118 reads how we measure reads. Amylase production by aspergillus niger through submerged. Effect of varying ph of the medium on amylase production ph amylase activity uml 5. Fungal species have been studied a lot for the production of alpha amylase because of the low cost of substrates used for the production of alpha amylases. Genetically modified organisms are also being used for production of. Paper open access effect of chemicals and cations on the. Submerged fermentation was carried out using muskmelon shell as a substrate for the production of amylase using aspergillus niger. Research article comparative study of the activity of. Glucose oxidase from a genetically modified strain of aspergillus niger dp. The effects of ethylene diamine tetratacetic acid edta, mercuric chloride hgcl 2, calcium ion ca. Amy is one of the enzymes with high demand for industrial applications.
Production and stabilization of amylases from aspergillus. Since residual amounts of the food enzyme are removed by distillation, dietary exposure was only calculated for brewing processes. Safety evaluation of the food enzyme amylase from the. Production and stabilization of amylases from aspergillus niger. Alpha amylase, aspergillus niger, substrate, solid state fermentation. It was observed that the activity started to peak at 60 hrs as 102. Effect of metal ions on enzyme activity metal ion play an important role for the production of. So activity can be further enhanced by lower concentrations of calcium, cobalt, magnesium and sodium ions. Abstract aspergillus niger ncim 820 was grown in rice bran since its a good carbon and starch source which has the ability to produce amylase. Thus the present study was designed in search of cheaper carbon sources for the production of alpha amylase enzyme by fungal strains. Thus, species of aspergillus such as aspergillus oryzae and aspergillus niger are studied extensively for industrial production of. Enhanced production of alpha amylase using vegetables wastes by aspergilllus niger strain sk01 marine isolate s. Bioprocesing of curcuma angustifolia for alpha amylase production by aspergillus niger.
Pdf submerged fermentation holds tremendous fungal potentiality in high biomass yield of alphaamylase. Use of 4 agroindustrial by products and organic materials as nitrogen sources for production of aspergillus oryzae s2. Alpha amylase produced using the two substrates showed good activity but banana peel as a substrate exhibited highest activity of 1. General description microbial amylases are exoenzymes and are used in several industrial applications, such as production of bread, maltose syrups, and fermentation of soya sauce, miso etc. In the present study a fungi was newly isolated from municipal dumping area of midnapore town, west bengal, india for the production of. Change in hyphal morphology of aspergillus oryzae during. A1185 alphaamylase from gm aspergillus niger as a processing aid enzyme page content the purpose of this application is to approve the use of alphaamylasee sourced from rhizomucor pusillus in gm aspergillus niger. Enhanced production of alpha amylase using vegetables. Amylase production by aspergillus niger through submerged fermentation using starchy food byproducts as substrate article pdf available october 2016 with 3,739 reads how we measure reads. Production of alphaamylase from aspergillus oryzae for. The optical growth of the isolate was found to be at 26 hours. The initial step in the digestion of starch occurs in the mouth with the secretion of. All the aspergillus niger isolates were tested for amylase production by starch hydrolysis.
Optimization of cultural conditions is of utmost importance for better production of alpha amylase. Characterization of extracellular amylase enzyme produced. Amylase production on submerged fermentation by bacillus. Pdf five fungal isolates were screened for the production of alphaamylase using both solidstate and submerged fermentations. Aspergillus fumigatus has been employed for production of the enzyme by submerged fermentation technique 15. Pdf bioprocesing of curcuma angustifolia for alpha. The enzyme was partially purified with activated carbon which gave a 1.
Alpha amylase was produced by aspergillus niger under submerged fermentation conditions in a basal salt medium. Thus, the present study was designed in the search of cheaper substrate for the production of alpha amylase enzyme by aspergillus niger. Optimization and production of fungal amylases using. The study aimed at isolation and screening of fungal amylase producer, optimization of solid state fermentation conditions for maximum amylase production by the best amylase producer, and characterization of the crude amylases, so produced. The highest and least alphaamylase production was found when 6 and 2 discs spore of a. The present study suggest that white yam water act as a potent substrate for industrial production of. Adharsh rajasekar, rahulsribalaji gandhiraj dhamodharan. Ravi kumar kadeppagari for the award of doctor of philosophy in biotechnology, to the university of mysore, is the result of research work carried out by.
The selected strain was studied for optimization of growth conditions for production of amylase. The cultural and nutrient requirements of aspergillus niger for production of. Production and characterization amylase from aspergillus. The purpose of optimization is to find out conditions under which the fungus can flourish luxuriantly and produce. Pdf amylase production by aspergillus niger through. Microbial growth and production of amylolytic enzymes by a. Fungal species have been studied a lot for the production of alpha amylase pandey a, nigam p et al. Four fungal isolates from soil were screened for alpha amylase production and the isolate.
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